programmable arbitrary waveform generator 33 220 Search Results


anti pdgf bb antibody  (R&D Systems)
92
R&D Systems anti pdgf bb antibody
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Anti Pdgf Bb Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti pdgf bb antibody/product/R&D Systems
Average 92 stars, based on 1 article reviews
anti pdgf bb antibody - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
male wistar rats  (Inotiv)
93
Inotiv male wistar rats
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Male Wistar Rats, supplied by Inotiv, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/male wistar rats/product/Inotiv
Average 93 stars, based on 1 article reviews
male wistar rats - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
function generator  (Agilent technologies)
90
Agilent technologies function generator
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Function Generator, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/function generator/product/Agilent technologies
Average 90 stars, based on 1 article reviews
function generator - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
anodic alumina membranes (aam)  (Synkera Technologies Inc)
90
Synkera Technologies Inc anodic alumina membranes (aam)
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Anodic Alumina Membranes (Aam), supplied by Synkera Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anodic alumina membranes (aam)/product/Synkera Technologies Inc
Average 90 stars, based on 1 article reviews
anodic alumina membranes (aam) - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
male sprague-dawley rats  (Charles River Laboratories)
90
Charles River Laboratories male sprague-dawley rats
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Male Sprague Dawley Rats, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/male sprague-dawley rats/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
male sprague-dawley rats - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
long-evans rats  (Charles River Laboratories)
90
Charles River Laboratories long-evans rats
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Long Evans Rats, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/long-evans rats/product/Charles River Laboratories
Average 90 stars, based on 1 article reviews
long-evans rats - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
lb-miller broth  (Difco)
90
Difco lb-miller broth
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Lb Miller Broth, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lb-miller broth/product/Difco
Average 90 stars, based on 1 article reviews
lb-miller broth - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
adjustable focus lens nikon ajp-heart  (Nikon)
90
Nikon adjustable focus lens nikon ajp-heart
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Adjustable Focus Lens Nikon Ajp Heart, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adjustable focus lens nikon ajp-heart/product/Nikon
Average 90 stars, based on 1 article reviews
adjustable focus lens nikon ajp-heart - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
ds en 12260 2003  (Shimadzu Corporation)
99
Shimadzu Corporation ds en 12260 2003
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Ds En 12260 2003, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ds en 12260 2003/product/Shimadzu Corporation
Average 99 stars, based on 1 article reviews
ds en 12260 2003 - by Bioz Stars, 2026-04
99/100 stars
  Buy from Supplier
luciferin buffer  (Thermo Fisher)
98
Thermo Fisher luciferin buffer
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Luciferin Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luciferin buffer/product/Thermo Fisher
Average 98 stars, based on 1 article reviews
luciferin buffer - by Bioz Stars, 2026-04
98/100 stars
  Buy from Supplier
uv-vis spectrophotometer evolution 220  (Thermo Fisher)
90
Thermo Fisher uv-vis spectrophotometer evolution 220
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Uv Vis Spectrophotometer Evolution 220, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/uv-vis spectrophotometer evolution 220/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
uv-vis spectrophotometer evolution 220 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
syringe pump  (World Precision Instruments)
96
World Precision Instruments syringe pump
Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) <t>PDGF-BB</t> concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.
Syringe Pump, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/syringe pump/product/World Precision Instruments
Average 96 stars, based on 1 article reviews
syringe pump - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

Image Search Results


Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) PDGF-BB concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.

Journal: Scientific reports

Article Title: Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts.

doi: 10.1038/srep28538

Figure Lengend Snippet: Figure 1. Synergetic effect of mixed sheets consisting of PBMNCs and fibroblasts. (a) Comparison of VEGF secretion. The VEGF concentration in the culture medium was measured in PBMNCs, fibroblast sheets, and mixed cell sheets for 3 days. Normoxic condition: 37 °C, 20% O2 for 3 days. Hypoxic condition: 37 °C, 20% O2 for 2 days followed by 33 °C, 2% O2 for 1 day. (b) Secretion from PBMNCs increased VEGF production by fibroblasts. Fibroblasts were cultured for 48 h with or without the PBMNC-conditioned medium, and the VEGF concentration in the supernatant was analyzed by ELISA. (c) TGF-β1 concentration in fibroblasts and PBMNC culture medium at 48 h. (d) PDGF-BB concentration in fibroblasts and PBMNC culture medium at 48 h. (e) Neutralizing antibody against TGF-β1 and PDGF-BB inhibited VEGF production in fibroblasts. The PBMNC-conditioned medium was co-cultured with a neutralizing antibody against TGF-β1 or PDGF-BB, and the PBMNC-conditioned medium was added to fibroblasts. After 48 h, the VEGF concentration was measured by ELISA. (f) TGF-β1 and PDGF-BB recombinant proteins elevated VEGF production by fibroblasts. (g) The PBMNC-conditioned medium increased the expression levels of VEGF, collagen I, collagen III, α-SMA, and Axin2 mRNA. Fibroblasts were cultured with the PBMNC-conditioned or control medium for 48 h. The mRNA expression levels were determined using real-time PCR. ACTB was used as an endogenous control. The expression levels were compared with that in control medium, which is presented as 1.

Article Snippet: In the neutralizing antibody experiment, the conditioned medium was incubated for 90 min at 4 °C with anti-ATM antibody (38 μ g/mL, ab78, Abcam, Cambridge, UK) as a control antibody, anti-TGF-β 1 antibody (38 μ g/mL, ab64715, Abcam), or anti-PDGF-BB antibody (33 μ g/mL, AF-220-NA, R&D Systems, Inc.) and then added to fibroblasts.

Techniques: Comparison, Concentration Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Recombinant, Expressing, Control, Real-time Polymerase Chain Reaction